Open Access Medical Books

GEL ELECTROPHORESIS – PRINCIPLES AND BASICS

GEL ELECTROPHORESIS
Edited by Sameh Magdeldin .
376 pages .

Even though there is a huge number of books and publications utilizing different 
aspects of separation techniques like gel electrophoresis, it is still hard to find a freely 
accessible book that gathers a solid and concise understanding of gel separation 
principles together with its applications. The vision of this book is to provide an open 
source book series demonstrating the concept of gel bio-separation with some of its 
applications that meets the current throughput screening demands of scientists and 
researchers. The book “Gel Electrophoresis – Principles and Basics” begins with an 
introductory chapter that describes the principles of well-known gel separation 
approaches using agarose and polyacrylamide matrices, together with snapshot
applications of this analytical technique. It is followed by wide-ranged practical 
research chapters utilizing widely popular techniques such as 2DE, DGGE, and PFGE, 
written by leading experts worldwide. It is safe to to say that the scope of information 
contained in this book is large and rich enough to be covered in a book series.
Gel electrophoresis is aimed mainly at those interested in different separation techniques, 
particularly biochemists, biologists, pharmacists, advanced graduate students and 
postgraduate researchers.
Finally, I am grateful to Ms Martina Durovic (publishing process manager) and all the 
experts who participated in this book and shared their valuable experience. Indeed, 
without their participation, this book wouldn’t have come to light.

Sameh Magdeldin, MVSc, PhD (Physiology), PhD (Proteomics),
Senior post doc researcher and Proteomics team leader,
Medical School, Niigata University, Japan,
Assistant Professor (Lecturer), Physiology Department,
Suez Canal University,
Egypt

CONTENTS : 

Part 1 Basic Principles of Gel Electrophoresis 1


Chapter 1 Introduction to Agarose and Polyacrylamide 
Gel Electrophoresis Matrices with Respect to Their Detection Sensitivities 3
Patricia Barril and Silvia Nates

Chapter 2 Gel-Electrophoresis and Its Applications 15
Pulimamidi Rabindra Reddy and Nomula Raju

Chapter 3 Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis 33
Muhittin Yılmaz, Cem Ozic and İlhami Gok

Chapter 4 Discriminatory Power of Agarose 
Gel Electrophoresis in DNA Fragments Analysis 41
Seow Ven Lee and Abdul Rani Bahaman

Chapter 5 Gel Electrophoresis of Proteins 57
Laura García-Descalzo, Eva García-López, Alberto Alcázar, Fernando Baquero and Cristina Cid

Chapter 6 Gel Electrophoresis of Protein 
– From Basic Science to Practical Approach 69
Gholamreza Kavoosi and Susan K. Ardestani

Part 2 Two Dimensional Polyacrylamide Gel Electrophoresis 89


Chapter 7 Two-Dimensional Polyacrylamide 
Gel Electrophoresis – A Practical Perspective 91
Sameh Magdeldin, Ying Zhang, Bo Xu, Yutaka Yoshida and Tadashi Yamamoto
Chapter 8 High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis: A Tool for Identification of Polymorphic and Modified Linker Histone Components 117
Andrzej Kowalski and Jan Pałyga

Chapter 9 Two-Dimensional Gel Electrophoresis (2-DE) 137
Bruno Baudin

Chapter 10 High Speed Isoelectric Focusing of Proteins Enabling Rapid Two-Dimensional Gel Electrophoresis 157
Gary B. Smejkal and Darren J. Bauer

Part 3 Denaturing Gradient Gel Electrophoresis (DGGE) 171


Chapter 11 Denaturing Gradient Gel Electrophoresis (DGGE) 
in Microbial Ecology – Insights from Freshwaters 173
Sofia Duarte, Fernanda Cássio and Cláudia Pascoal

Part 4 Statistical and Bioinformatic Analysis of Electrophoresis Data 197


Chapter 12 Statistical Analysis of Gel Electrophoresis Data 199
Kimberly F. Sellers and Jeffrey C. Miecznikowski

Chapter 13 Quantitative Analysis of Electrophoresis Data – Application 
to Sequence-Specific Ultrasonic Cleavage of DNA 217
Sergei Grokhovsky, Irina Il’icheva, Dmitry Nechipurenko, Michail Golovkin, Georgy Taranov, Larisa Panchenko, Robert Polozov and Yury Nechipurenko

Part 5 Pulsed Field Gel Electrophoresis (PFGE) 239


Chapter 14 The Use of Pulsed Field Gel Electrophoresis 
in Listeria monocytogenes Sub-Typing –
Harmonization at the European Union Level 241
Benjamin Félix, Trinh Tam Dao, Bertrand Lombard, Adrien Asséré Anne Brisabois and Sophie Roussel

Part 6 Bacterial Electrophoretic Techniques 255


Chapter 15 Electrophoretic Techniques in Microbial Ecology 257
Elena González-Toril, David Lara-Astiaso, Ricardo Amils and Angeles Aguilera

Chapter 16 Application of Multiplex PCR, 
Pulsed-Field Gel Electrophoresis (PFGE), and BOX-PCR for Molecular Analysis of Enterococci 269
Charlene R. Jackson, Lori M. Spicer, John B. Barrett and Lari M. Hiott

Chapter 17 The Use of Pulsed Field Gel Electrophoresis in Listeria 
monocytogenes Sub-Typing – Comparison with MLVA Method Coupled with Gel Electrophoresis 299
Sophie Roussel, Marie-Léone Vignaud, Jonass T Larsson, Benjamin Félix, Aurore Rossignol, Eva Moller Nielsen and Anne Brisabois

Chapter 18 Restriction Fragment Length Polymorphism 
Analysis of PCR-Amplified Fragments (PCR-RFLP) and Gel Electrophoresis – Valuable Tool for Genotyping and Genetic Fingerprinting 315
Henrik Berg Rasmussen

Chapter 19 Application of Two-Dimensional 
Gel Electrophoresis to Microbial Systems 335
Fatemeh Tabandeh, Parvin Shariati and Mahvash Khodabandeh .


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