Open Access Medical Books



Edited by Ingrid Schmid .

214 pages . 
Open Access . 

Advances in patient management have often been closely linked to the development of critical quantitative analysis methods. Flow cytometry is such an important methodology. It can be applied to individual cells or organelles allowing investigators interested in obtaining information about the functional properties of cells to assess the differences among cells in a heterogeneous cell preparation or between cells from separate samples. It is characterized by the use of a select wavelength of light (or multiple ones) to interrogate cells or other particles one at a time providing statistically relevant, rapid correlated measurements of multiple parameters with excellent temporal resolution. These intrinsic attributes, as well as advances in instrumentation and fluorescent probes and reagents, have contributed to the tremendous growth of clinical applications of flow cytometry and to the world-wide expansion of laboratories which use this technology since its inception in the late 1960s.
This publication reflects these facts as indicated by the global author panel and the wide range of sample types, assays, and methodologies described. Openly accessible, the book is intended to introduce novices to this powerful technology and also provide experienced professionals with valuable insights and an opportunity to refresh or update their knowledge in various subject areas of clinical flow cytometry.

Ingrid Schmid, Mag. Pharm.
Department of Medicine
Division of Hematology-Oncology
University of California, Los Angeles


1 Effect of Monocyte Locomotion Inhibitory 
Factor (MLIF) on the Activation and Production of
Intracellular Cytokine and Chemokine Receptors in Human T CD4+ Lymphocytes Measured by Flow Cytometry 1
Sara Rojas-Dotor

2 Applications of Flow Cytometry to Clinical Microbiology 17
Barbara Pieretti, Annamaria Masucci and Marco Moretti

3 High-Throughput Flow Cytometry for Predicting Drug-Induced Hepatotoxicity 43
Marion Zanese, Laura Suter, Adrian Roth, Francesca De Giorgi and François Ichas

4 B Cells in Health and Disease – Leveraging Flow Cytometry 
to Evaluate Disease Phenotype and the Impact of Treatment with Immunomodulatory Therapeutics 60
Cherie L. Green, John Ferbas and Barbara A. Sullivan

5 Evaluation of the Anti-Tumoural and Immune Modulatory 
Activity of Natural Products by Flow Cytometry 91
Susana Fiorentino, Claudia Urueña, Sandra Quijano, Sandra Paola Santander,
John Fredy Hernandez and Claudia Cifuentes

6 Identification and Characterization of 
Cancer Stem Cells Using Flow Cytometry 107
Yasunari Kanda

7 Flow Based Enumeration of Plasmablasts in Peripheral Blood After Vaccination as a Novel
Diagnostic Marker for Assessing Antibody Responses in Patients with Hypogammaglobulinaemia 125
Vojtech Thon, Marcela Vlkova, Zita Chovancova, Jiri Litzman and Jindrich Lokaj

8 Applications of Flow Cytometry in 
Solid Organ Allogeneic Transplantation 143
Dimitrios Kirmizis, Dimitrios Chatzidimitriou, Fani Chatzopoulou, Lemonia Skoura and Gregory Myserlis

9 The Use of Flow Cytometry to Monitor T Cell Responses 
in Experimental Models of Graft-Versus-Host Disease 151
Bryan A. Anthony and Gregg A. Hadley

10 Lymphocyte Apoptosis, Proliferation and Cytokine Synthesis 
Pattern in Children with Helicobacter pylori Infection 173
Anna Helmin-Basa, Lidia Gackowska, Izabela Kubiszewska, Malgorzata Wyszomirska-Golda,
Andrzej Eljaszewicz, Grazyna Mierzwa, Anna Szaflarska-Poplawska, Mieczyslawa Czerwionka-Szaflarska, Andrzej Marszalek and Jacek Michalkiewicz

11 The Effect of Epigallocatechin Gallate (EGCG) and Metal Ions Corroded from Dental Casting Alloys on Cell Cycle Progression and Apoptosis in Cells from Oral Tissues 191
Jiansheng Su, Zhizen Quan, Wenfei Han, Lili Chen and Jiamei Gu .

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Published by: Unknown - Monday, March 11, 2013


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