Open Access Medical Books

TEXTBOOK : FLOW CYTOMETRY – RECENT PERSPECTIVES

FLOW CYTOMETRY

Edited by Ingrid Schmid .

510 pages . 
Open Access .

Advances in cell biology have always been closely linked to the development of critical quantitative analysis methods. Flow cytometry is such a pivotal methodology. It can be applied to individual cells or organelles allowing investigators interested in obtaining information about the functional properties of cells to assess the differences among cells in a heterogeneous cell preparation or between cells from separate samples. It is characterized by the use of a select wavelength of light (or multiple ones) to interrogate cells or other particles one at a time providing statistically relevant, rapid correlated measurements of multiple parameters with excellent temporal resolution. These intrinsic attributes, as well as advances in instrumentation and fluorescent probes and reagents, have contributed to the tremendous growth of applications in flow cytometry and to the world-wide expansion of laboratories which use this technology since its inception in the late 1960s.
This publication reflects these facts as indicated by the global author panel and the wide range of sample types, assays, and methodologies described. The compendium will give readers a historical perspective as well as the current state of important topics in flow cytometry. Openly accessible, the book is intended to introduce novices to this powerful technology and also provide experienced professionals with valuable insights and an opportunity to refresh or up-date their knowledge in various subject areas of flow cytometry.

Ingrid Schmid, Mag. pharm.
Department of Medicine
Division of Hematology-Oncology
University of California, Los Angeles
USA

CONTENTS OF THE TEXTBOOK :


1 What Flow Cytometry Can 
Tell Us About Marine Micro-Organisms – Current Status and Future Applications 1
A. Manti, S. Papa and P. Boi

2 Identification and Characterisation of Microbial 
Populations Using Flow Cytometry in the Adriatic Sea 29
Danijela Šantić and Nada Krstulović

3 Flow Cytometry as a Powerful Tool for 
Monitoring Microbial Population Dynamics in Sludge 43
Audrey Prorot, Philippe Chazal and Patrick Leprat

4 Flow Cytometry Applications in Food Safety Studies 69
Antonello Paparella, Annalisa Serio and Clemencia Chaves López

5 Estimation of Nuclear DNA Content and 
Determination of Ploidy Level in Tunisian Populations of Atriplex halimus L. by Flow Cytometry 103
Kheiria Hcini, David J. Walker, Elena González, Nora Frayssinet, Enrique Correal and Adok S. Bouzid

6 Yeast Cell Death During 
the Drying and Rehydration Process 119
Boris Rodríguez-Porrata, Didac Carmona-Gutierrez, Gema López-Matínez, Angela Reisenbichler, Maria Bauer, Frank Madeo and Ricardo Cordero-Otero

7 Use of Flow Cytometry in the In Vitro and In Vivo Analysis 
of Tolerance/Anergy Induction by Immunocamouflage 133
Duncheng Wang, Wendy M. Toyofuku, Dana L. Kyluik and Mark D. Scott

8 Multiplexed Cell-Counting Methods by Using 
Functional Nanoparticles and Quantum Dots 151
Hoyoung Yun, Won Gu Lee and Hyunwoo Bang

9 Implementation of a Flow Cytometry Strategy to Isolate 
and Assess Heterogeneous Membrane Raft Domains 169
Morgan F. Khan, Tammy L. Unruh and Julie P. Deans

10 Broad Applications of Multi-Colour 
Time-Resolved Flow Cytometry 185
Ben J. Gu and James S. Wiley

11 Application of Flow Cytometry 
in the Studies of Microparticles 203
Monika Baj-Krzyworzeka, Jarek Baran, Rafał Szatanek and Maciej Siedlar

12 Flow Cytometry-Based Analysis 
and Sorting of Lung Dendritic Cells 237
Svetlana P. Chapoval

13 Stem Cell Characterization 261 
Arash Zaminy

14 Flow Cytometric Sorting of Cells from Solid 
Tissue – Reagent Development and Application 283
P. S. Canaday and C. Dorrell

15 Experimental Conditions and Mathematical 
Analysis of Kinetic Measurements Using
Flow Cytometry – The FacsKin Method 299
Ambrus Kaposi, Gergely Toldi, Gergő Mészáros, Balázs Szalay, Gábor Veress and Barna Vásárhelyi

16 Analysis of Cellular Signaling 
Events by Flow Cytometry 325
Jacques A. Nunès, Guylène Firaguay and Emilie Coppin

17 Gamma Radiation Induces p53-Mediated 
Cell Cycle Arrest in Bone Marrow Cells 337
Andrea A. F. S. Moraes, Lucimar P. França, Vanina M. Tucci-Viegas, Fernanda Lasakosvitsch,
Silvana Gaiba, Fernanda L. A. Azevedo, Amanda P. Nogueira, Helena R. C. Segreto, Alice T. Ferreira and Jerônimo P. França

18 Early Events in Apoptosis Induction 
in Polymorphonuclear Leukocytes 357
Annelie Pichert, Denise Schlorke, Josefin Zschaler, Jana Fleddermann, Maria Schönberg, Jörg Flemmig and Jürgen Arnhold

19 New Insights into Cell Encapsulation and the Role of Proteins During Flow Cytometry 373
Sinéad B. Doherty and A. Brodkorb

20 Median Effect Dose and Combination Index 
Analysis of Cytotoxic Drugs Using Flow Cytometry 393
Tomás Lombardo, Laura Anaya, Laura Kornblihtt and Guillermo Blanco

21 Flow Cytometry Analysis of Intracellular Protein 421 . 
 Irena Koutná, Pavel Šimara, Petra Ondráčková and Lenka Tesařová

22 Biological Effects Induced by 
Ultraviolet Radiation in Human Fibroblasts 439
Silvana Gaiba, Vanina M. Tucci-Viegas, Lucimar P. França, Fernanda Lasakosvitsch, Fernanda L. A. Azevedo, Andrea A. F. S. Moraes, Alice T. Ferreira and Jerônimo P. França

23 Immunophenotypic Characterization 
of Normal Bone Marrow Stem Cells 457
Paula Laranjeira, Andreia Ribeiro, Sandrine Mendes, Ana Henriques, M. Luísa Pais and Artur Paiva

24 Ethanol Extract of Tripterygium wilfordii Hook. f. 
Induces G0/G1 Phase Arrest and Apoptosis in Human Leukemia HL-60 Cells Through c-Myc and Mitochondria-Dependent Caspase Signaling Pathways 479
Jai-Sing Yang, Yun-Peng Chao, Li-Jen Lin, Wen-Wen Huang, Jing-Gung Chung, Shu-Fen Peng, Chi Cheng Lu, Jo-Hua Chiang, Shu-Ren Pai, Minoru Tsuzuki and Chung-Jen Chiang

25 Applications of Quantum Dots in Flow Cytometry 491
Dimitrios Kirmizis, Fani Chatzopoulou, Eleni Gavriilaki and Dimitrios Chatzidimitriou .


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