Open Access Medical Books


Edited by Gregory G. Brown .

336 pages . 
Open Access .

The development of technology in the early 1970s for propagating targeted segmentsof DNA in bacterial plasmids and viruses, molecular cloning, created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide ample evidence of just how extensive the applications of molecular cloning have become. The chapters of this have been organized largely according to the fields this technology is being applied.
Two chapters deal with the recent advances in molecular cloning technology per se. Padmanabhan and colleagues review various methods for cloning in E. coli plasmid vectors, emphasizing the shortcomings of various procedures for identifying clones of interest. Abbas Padeganeh et al. provide an interesting discussion of non-viral systems for gene delivery into mammalian cells, with an emphasis on the relatively new “dendrosome” technology.
Several chapters deal with the use of molecular cloning techniques for obtaining and characterizing purified animal proteins involved in cancer and aspects of cell biology.
The proteins thus characterized include human cathepsins (Wen et al.), a human WAP-like domain (Jayanthi et al.) and potential antibiotic peptides from amphibian skin secretions (Zhao et al.). Three chapters, those of Sonoki, Crespo et al. and Esteso et al., deal with the applications of molecular cloning methodologies to improving our understanding of immune system, while the chapter by Hanif and colleagues deals with the use of the methodology for the production of antigenic peptides and vaccines.
Applications in the area of toxicology are reviewed in the chapter by Radis-Baptista, while more specific application of the technology to the purification and characterization of a toxic enzyme from spider venom is covered in the chapter by Tambourgi et al. The contributions of Ogbunude et al. and Jarilla et al. describe the cloning and expression of potential therapeutic targets for trypanosomal and trematode parasites, respectively, while Shahein et al. describe the use of whole genome sequences as a means of developing anti-parasitic vaccines. Liu et al. and Chen et al. describe applications to phylogenetic questions. Finally, two contributions in the area of plant biology are described. Sottomayor et al. describe how molecular cloning technology can be used to understand the complicated pathway by which the anti-cancer terpenoid indole alkaloids vineblastine and vincristine are synthesized, while Brown and Gaborieau discuss the application of positional cloning with the complex genomes of polyploid plants.
There is, in short, something for a wide variety of readers in a truly diverse set of scientific fields.

Gregory G. 
BrownMcGill University, Montreal QC,


Part 1 of the textbook : Technological Advances .

1 Screening of Bacterial Recombinants: Strategies and Preventing False Positives 3 Sriram Padmanabhan, Sampali Banerjee and Naganath Mandi

2 Non-Viral Vehicles: Principles,  Applications, and Challenges in Gene Delivery 21  Abbas Padeganeh, Mohammad Khalaj-Kondori,  
Babak Bakhshinejad and Majid Sadeghizadeh

Part 2 of the textbook : Cancer and Cell Biology .

3 Subcloning and Expression of Functional Human Cathepsin B and K in E. coli: Characterization and Inhibition by Flavonoids 37  
Lisa Wen, Soe Tha, Valerie Sutton,  
Keegan Steel, Franklin Rahman,  
Matthew McConnell, Jennifer Chmielowski,  
Kenneth Liang, Roxana Obregon, Jessica LaFollette,  
Laura Berryman, Ryan Keefer,  
Michael Bordowitz, Alice Ye,  
Jessica Hunter, Jenq-Kuen Huang and Rose M. McConnell

4 Molecular Cloning and Overexpression of  WAP Domain of Anosmin-1 (a-WAP) in Escherichia coli 59  Srinivas Jayanthi, Beatrice Kachel, Jacqueline Morris,  
Igor Prudovsky and Thallapuranam K. Suresh Kumar

5 Effects of Two Novel Peptides  from Skin of Lithobates Catesbeianus on  Tumor Cell Morphology and Proliferation 73  
Rui-Li ZHAO, Jun-You HAN, Wen-Yu HAN,  
Hong-Xuan HE and Ji-Fei MA

Part 3 of the textbook : Immunology/Hematology .

6 Molecular Cloning of Immunoglobulin Heavy  Chain Gene Translocations by Long Distance Inverse PCR 83  Takashi Sonoki

7 Identification of Molecules Involved in the Vulture  Immune Sensing of Pathogens by Molecular Cloning 91  Elena Crespo, José de la Fuente and José M. Pérez de la Lastra

8 Molecular Cloning, Characterization,  Expression Analysis and Chromosomal  Localization of the Gene Coding for the Porcine  
αIIb Subunit of the αIIbβ3 Integrin Platelet Receptor 109  
Gloria Esteso, Ángeles Jiménez-Marín,  
Gema Sanz, Juan José Garrido and Manuel Barbancho

9 Molecular Cloning, Expression,  Purification and Immunological  Characterization of Proteins Encoded by Regions  
of Difference Genes of Mycobacterium tuberculosis 141  
Shumaila Nida Muhammad Hanif,  
Rajaa Al-Attiyah and Abu Salim Mustafa

Part 4 of the textbook : Toxicology .

10 Molecular Toxinology – Cloning Toxin Genes for Addressing  Functional Analysis and Disclosure Drug Leads 161  Gandhi Rádis-Baptista

11 Molecular Cloning, Expression, Function,  Structure and Immunoreactivities of a  Sphingomyelinase D from Loxosceles adelaida,  a Brazilian Brown Spider from Karstic Areas 197 
Denise V. Tambourgi, Giselle Pidde-Queiroz,  
Rute M. Gonçalves-de-Andrade, Cinthya K. Okamoto, 
Tiago J. Sobreir, Paulo S. L. de Oliveira,  
Mário T. Murakami and Carmen W. van den Berg

Part 5 of the textbook : Parasitology .

12 Cloning the Ribokinase of  Kinetoplastidae: Leishmania Major 221  Patrick Ogbunude, Joy Ikekpeazu,  
Joseph Ugonabo, Michael Barrett and Patrick Udeogaranya

13 Genome Based Vaccines Against Parasites 231  Yasser Shahein and Amira Abouelella

14 Phosphagen Kinase System of the  Trematode Paragonimus westermani: Cloning and  Expression of a Novel Chemotherapeutic Target 247  Blanca R. Jarilla and Takeshi Agatsuma

Part 6 of the textbook : Evolutionary Biology .

15 Molecular Cloning, Expression Pattern, and  Phylogenetic Analysis of the Lysyl-tRNA Synthetase Gene  from the Chinese Oak Silkworm Antheraea pernyi 267  Yan-Qun Liu and Li Qin

16 Molecular Cloning and Characterization of  Fe-Superoxide Dismutase (Fe-SOD) from  the Fern Ceratopteris thalictroides 277  Chen Chen and Quanxi Wang

Part 7 of the textbook : Plant Biology .

17 Cloning and Characterization of a Candidate Gene  from the Medicinal Plant Catharanthus roseus  Through Transient Expression in Mesophyll Protoplasts 291  Patrícia Duarte, Diana Ribeiro, Gisela Henriques,  Frédérique Hilliou, Ana Sofia Rocha, Francisco Lima,  Isabel Amorim and Mariana Sottomayor

18 Positional Cloning in Brassica napus: Strategies for  Circumventing Genome Complexity in a Polyploid Plant 309  Gregory G. Brown and Lydiane Gaborieau .

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Published by: Unknown - Wednesday, March 13, 2013


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