Open Access Medical Books

TEXTBOOK : GENETIC TRANSFORMATION

GENETIC TRANSFORMATION

Edited by María Alejandra Alvarez .

340 pages .
Open Access . 

It has been more than twenty five years now since the first transformed plant was reported. Plant transgenesis has evolved since those first attempts; the advances have led to the elucidation of numerous aspects of plant biology, physiology, and Agrobacterium biology. Even more spectacular is the impact of plant transformation on crop improvement. To this date, transgenic crops represent a 10 % of the 1.5 billion hectares of cropland worldwide, and constitute one of the main sources of incomes for countries like the USA (66.8 million hectares), Brazil (25.4), Argentina (22.9), India (9.4), Canada (8.8), China (3.5), Paraguay (2.6), Pakistan (2.4), South Africa (2.2) and Uruguay with 1.1 million hectares. Also, plant transformation had influence on fruit and forest improvement and the development of desirable traits for ornamental plant breeders. Moreover, the significant advances made in the use of transformed plants for the production of recombinant proteins and for the engineering of secondary metabolism pathways have made a new easy-scalable and economical rendering platform available to the pharmaceutical industry.
Chapters in this book represent selected examples of the advances that are currently undergoing in this field. In the first section, the history and progress of Agrobacterium utilization as a transformation vector is presented along with a chapter dedicated to the analysis of the related events from a structure-functional analysis. Also the physiological and molecular background of phytoremediation is analyzed.
In the second section, the subject of plant improvement is widely covered in the chapters related to the amelioration of crops, fruits and flowers with a couple of chapters dedicated to the last advances in molecular farming and RNAi technology.
Finally, in the last section the paramount contribution that plant transformation has made on secondary metabolism is reviewed.
I would like to thank each of the authors for their great efforts in producing their articles. I am sure the readers will appreciate the contribution each of the researchers has made and will recognize the value of each chapter.
Finally, I would like to thank the staff of the InTech Open Access Publisher for their invaluable support along the process of publishing this book, particularly Ms. Dragana Manestar and Ms. Natalia Reinic.


Dr. María Alejandra Alvarez
National Scientific and Technical Research Council (CONICET)
Buenos Aires
Argentina

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CONTENTS :

Part 1 of the textbook : Agrobacterium: New Insights into a Natural Engineer .


1 Agrobacterium-Mediated Genetic Transformation: History and Progress 3 Minliang Guo, Xiaowei Bian, Xiao Wu and Meixia Wu

2 Structure-Function Analysis of Transformation Events 29 Yuri N. Zhuravlev and Vladik A. Avetisov




Part 2 of the textbook : Plant Transformation: Improving Quality of Fruits, Crops and Trees - Molecular Farming .


3 Genetic Transformation in Tomato: Novel Tools to Improve Fruit Quality and Pharmaceutical Production 55 Antonio Di Matteo, Maria Manuela Rigano, Adriana Sacco, Luigi Frusciante and Amalia Barone

4 Genetic Transformation Strategies in Fruit Crops 81 Humberto Prieto


5 Citrus Transformation: Challenges and Prospects 101 Vicente Febres, Latanya Fisher, Abeer Khalaf and Gloria A. Moore


6 Evaluation of Factors Affecting European Plum (Prunus domestica L.) Genetic Transformation 123 Yuan Song, Fatih Ali Canli, Farida Meerja, Xinhua Wang, 
Hugh A. L. Henry, Lizhe An and Lining Tian


7 Genetic Transformation of Wheat:  Advances in the Transformation Method and Applications  for Obtaining Lines with Improved Bread-Making Quality  
and Low Toxicity in Relation to Celiac Disease 135  
Javier Gil-Humanes, Carmen Victoria Ozuna, Santiago Marín,  
Elena León, Francisco Barro and Fernando Pistón



8 Maize Transformation to Obtain Plants  Tolerant to Viruses by RNAi Technology 151  Newton Portilho Carneiro and Andréa Almeida Carneiro


9 Genetic Transformation  of Triticeae Cereals for Molecular Farming 171  Goetz Hensel

10 Genetic Transformation of Forest Trees 191  Osvaldo A. Castellanos-Hernández, Araceli Rodríguez-Sahagún,  Gustavo J. Acevedo-Hernández and Luis R. Herrera-Estrella

11 Agrobacterium-Mediated Transformation  of Indonesian Orchids for Micropropagation 215  Endang Semiarti, Ari Indrianto, Aziz Purwantoro,  
Yasunori Machida and Chiyoko Machida


12 Transient Transformation of Red Algal Cells:  Breakthrough Toward Genetic Transformation  of Marine Crop Porphyra Species 241  
Koji Mikami, Ryo Hirata, Megumu Takahashi,  
Toshiki Uji and Naotsune Saga





Part 3 of the textbook : Plant Transformation as a Tool for Regulating Secondary Metabolism .


13 Application of Agrobacterium Rol Genes  in Plant Biotechnology: A Natural Phenomenon  of Secondary Metabolism Regulation 261  
Victor P Bulgakov, Yuri N Shkryl, Galina N Veremeichik,  
Tatiana Y Gorpenchenko and Yuliya V Inyushkina


14 Transformed Root Cultures of Solanum dulcamara L.:  A Model for Studying Production  of Secondary Metabolites 271  
Amani M. Marzouk, Stanley G. Deans,  
Robert J. Nash and Alexander I. Gray


15 Genetic Transformation  for Metabolic Engineering of Tropane Alkaloids 291  María Alejandra Alvarez and Patricia L. Marconi

16 Transgenic Plants for Enhanced  Phytoremediation – Physiological Studies 305  Paulo Celso de Mello- Farias, Ana Lúcia Soares Chaves 
and Claiton Leoneti Lencina .






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