Open Access Medical Books



Edited by Leonardo de Azevedo Calderon .

438 pages .
Open Access .
ISBN 978-953-51-0813-9 .

Since its invention by the Russian botanist Mikhail Semyonovich Tsvet in 1901 [1], chromatography has evolved into a flexible analytical technique of which there are many permutations with various applications both in academia and industry, and is considered the most versatile of all methods of chemical analysis. Chromatography is used in the separation of compounds according to their distribution between two phases.
The compound mixture is dissolved in a fluid known as mobile phase, which carries it through a structure holding another material known as stationary phase. The various constituents of the compound mixture travel at different speeds due to differences in the compound's partition coefficient which allows the separation based on differential partitioning between the two phases resulting in differential retention on the stationary phase, thus performing the separation. Nowadays, the use of chromatography is associated with a wide range of detection systems, including electrochemical, photometric and mass spectrometry, and plays a vital role in the advancement of science. The authors of Chromatography - the Most Versatile Method of Chemical Analysis have contributed chapters which focus on purification, analysis, models, retention parameters and sample preparation with different applications in biotechnology, ecology, environment, food and toxicology. Finally, I am most happy to have received contributions from internationally renowned contributors from different parts of the world join us to report on their traditional and innovative approaches, as well as reviews of the most relevant and impacting aspects of chromatography. I hope that readers of this book will find new ideas, approaches and inspiration to solve separation problems.
Finally, I would like to thank all the authors and Mr. Oliver Kurelic for their contributions and their cooperation throughout the previous year.

Leonardo de Azevedo Calderon
Centro de Estudos de Biomoléculas Aplicadas a Saúde
Universidade Federal de Rondônia
Fundação Oswaldo Cruz , Porto Velho,


 1 Purification of Phospholipases A2 from American Snake Venoms 1 Rodrigo G. Stábeli, Rodrigo Simões-Silva, Anderson M. Kayano, Gizeli S. Gimenez, Andrea A. Moura, Cleópatra A. S. Caldeira, Antonio Coutinho-Neto, Kayena D. Zaqueo, Juliana P. Zuliani, Leonardo A. Calderon and Andreimar M. Soares

2 Use of Chromatography in Animal Ecology 35 Emma B. Casanave, M. Soledad Araujo and Gustavo H. López

 3 2D-NanoLC-ESI-MS/MS for Separation and Identification of Mouse Brain Membrane Proteins 63 Phan Van Chi and Nguyen Tien Dung

 4 Analytical Methods for Quantification of Drug Metabolites in Biological Samples 79 Robert Roškar and Tina Trdan Lušin

 5 Current Trends in Sample Treatment Techniques for Environmental and Food Analysis 127 Paolo Lucci, Deborah Pacetti, Oscar Núñez and Natale G. Frega

 6 Using High Performance Liquid Chromatography (HPLC) for Analyzing Feed Additives 165 Jolanta Rubaj, Waldemar Korol and Grażyna Bielecka

 7 Analysis of Surfactants in Environmental Samples by Chromatographic Techniques 187 Juan M. Traverso-Soto, Eduardo González-Mazo and Pablo A. Lara-Martín

 8 Application of HPLC Analysis of Medroxyprogesterone Acetate in Human Plasma 217 Saksit Chanthai and Thanee Tessiri

 9 Chromatographic Analysis of Nitrogen Utilization and Transport in Arbuscular Mycorrhizal Fungal Symbiosis 231 Jin HaiRu and Jiang Xiangyan

 10 Instrumental Analysis of Tetrodotoxin 245 Manabu Asakawa, Yasuo Shida, Keisuke Miyazawa and Tamao Noguchi

 11 Chromatography as the Major Tool in the Identification and the Structure-Function Relationship Study of Amylolytic Enzymes from Saccharomycopsis Fibuligera R64 271 Wangsa T. Ismaya, Khomaini Hasan, Toto Subroto, Dessy Natalia and Soetijoso Soemitro

 12 Isolation and Purification of Sperm Immobilizing/Agglutinating Factors from Bacteria and Their Corresponding Receptors from Human Spermatozoa 295 Vijay Prabha and Siftjit Kaur

 13 Purification of Azurin from Pseudomonas Aeuroginosa 311 Sankar Ramachandran, Moganavelli Singh and Mahitosh Mandal

 14 Characterization of Apple Pectin – A Chromatographic Approach 325 Maria Helene Giovanetti Canteri, Alessandro Nogueira, Carmen Lúcia de Oliveira Petkowicz and Gilvan Wosiacki

 15 Chromatographic Retention Parameters as Molecular Descriptors for Lipophilicity in QSA(P)R Studies of Bile Acid 343 Mihalj Poša

 16 The Use of Solvation Models in Gas Chromatography 365 Fabrice Mutelet

 17 A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis 385 Biljana Nigović, Ana Mornar and Miranda Sertić .

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Published by: Unknown - Sunday, June 30, 2013


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