Open Access Medical Books


Edited by Fatima Shad Kaneez .
368 pages .
Open Access .
ISBN 978-953-51-0406-3 .

This book deals with the understanding of endogenous mechanisms of cells and their receptors as well as advantages of using this technique. It covers the basic principles and preparation types and also deals with the latest developments in the traditional patch clamp technique. Some chapters in this book take the technique to a next level of modulation and novel approach.
The patch-clamp technique was developed by Neher and Sakmann in 1976 as an effective refinement of the voltage-clamp-assay that demonstrated the function of single ion channels in fundamental cellular process. The patch-clamp technique has revolutionized the study of ion channels in a wide variety of cells.
This book has seventeen chapters and has been divided into two sections. First section deals with the exploration of underlying mechanisms of cellular activities using patch clamp technique. The second section deals with the advantages of this system.
The first section begins with a chapter on the black box properties of the system followed by how intracellular signalling pathways integrate the pore associated to P2X7 receptor with other large pores. Next chapter deals with combine usage of whole-cell patch and focal stimulation technique to study of single synapse of central nervous system. This is followed by the chapter on single-channel properties and pharmacological characteristics of KATP channels in primary afferent neurons.
Chapters five and six explore how renal potassium channels are regulated by protein kinases and phosphatases and characteristic features of BK channels respectively.
Then there are papers on "onion-peeling" technique, mitochondrial channels, membrane transport in red blood cells and electrical properties in Leishmaniamacrophage membrane.
Seven chapters under section entitled “Advantages of using patch clamp technique” describe how this electrophysiological method is found to be useful in studying cardiac channelopathies, gating charge movements, off target effects of clinically used drugs, Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) in Modulating Vascular Smooth Muscle Cells, Perforated Patch Clamp in non-neuronal cells, Drug screening and drug safety evaluation and antimicrobial peptides and viroporins.
This book will be of good value for students of physiology, neuroscience, cell biology and biophysics.

Dr. Fatima Shad Kaneez
Professor Human Physilogy
PAP RSB Institute of Health Sciences
Universiti Brunei Darussalam
Brunei Darussalam


Part 1 Exploring Cellular Mechanisms Using Patch Clamp Technique .

 1 Patch ClampTechnique for Looking at Serotonin Receptors in B103 Cell Lines: A Black Box Test 3 K. Fatima-Shad and K. Bradley

 2 Intracellular Signaling Pathways Integrating the Pore Associated with P2X7R Receptor with Other Large Pores 37 L.G.B. Ferreira, R.A.M. Reis, L.A. Alves and R.X. Faria

 3 Patch Clamp Study of Neurotransmission at Single Mammalian CNS Synapses 55 Norio Akaike

 4 Single-Channel Properties and Pharmacological Characteristics of KATP Channels in Primary Afferent Neurons 73 Takashi Kawano

 5 Regulation of Renal Potassium Channels by Protein Kinases and Phosphatases 91 Manabu Kubokawa

 6 BK Channels – Focus on Polyamines, Ethanol/Acetaldehyde and Hydrogen Sulfide (H2S) 109 Anton Hermann, Guzel F. Sitdikova and Thomas M. Weiger

 7 From Action Potential-Clamp to "Onion-Peeling" Technique – Recording of Ionic Currents Under Physiological Conditions 143 Ye Chen-Izu, Leighton T. Izu, Peter P. Nanasi and Tamas Banyasz

 8 Electrophysiological Techniques for Mitochondrial Channels 163 Rainer Schindl and Julian Weghuber

 9 Patch-Clamp Analysis of Membrane Transport in Erythrocytes 171 Guillaume Bouyer, Serge Thomas and Stéphane Egée

 10 Electrical Membrane Properties in the Model Leishmania-Macrophage 203 Marcela Camacho

Part 2 Advantages of Using Patch Clamp Technique .

 11 Cardiac Channelopathies: Disease at the Tip of a Patch Electrode 233 Brian P. Delisle

 12 Gating Charge Movement in Native Cells: Another Application of the Patch Clamp Technique 255 Oscar Vivas, Isabel Arenas and David E. García

 13 Use of Patch Clamp Electrophysiology to Identify Off-Target Effects of Clinically Used Drugs 267 Lioubov I. Brueggemann and Kenneth L. Byron

 14 Role of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) in Modulating Vascular Smooth Muscle Cells by Activating Large-Conductance Potassium Ion Channels 283 Donald K. Martin, Christopher G. Schyvens, Kenneth R. Wyse, Jane A. Bursill, Robert A. Owe-Young, Peter S. Macdonald and Terence J. Campbell

 15 Perforated Patch Clamp in Non-Neuronal Cells, the Model of Mammalian Sperm Cells 301 Jorge Parodi and Ataúlfo Martínez-Torres

 16 Drug Screening and Drug Safety Evaluation by Patch Clamp Technique 311 Yan Long and Zhiyuan Li

 17 Enhanced Patch-Clamp Technique to Study Antimicrobial Peptides and Viroporins, Inserted in a Cell Plasma Membrane with Fully Inactivated Endogenous Conductances 329 Marco Aquila, Mascia Benedusi, Alberto Milani and Giorgio Rispoli .

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Published by: Unknown - Wednesday, June 26, 2013


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