Open Access Medical Books

PROTEIN PURIFICATION

TEXTBOOK : PROTEIN PURIFICATION

Edited by Rizwan Ahmad .

Open Access . 
232 pages .
ISBN 978-953-307-831-1 .


Protein purification is essential for characterization of the function, structure and interactions of a protein of interest. The magnitude of the challenge for protein purification becomes clearer when one considers the variety of macromolecules present in a cell extract. The development of techniques and methods for protein purification has been an key prerequisite for many of the advancements made in biotechnology.
The completion of the human genome project and the development of highthroughput approaches indicates a dramatic acceleration in the pace of biological sciences. In medical research, purified proteins are used for a wide range of applications, including assay development, molecular biology applications, production of antibodies, binding studies and more. Indeed, protein purification is a first stage of almost all in vitro protein studies.
The present volume on “Protein Purification” aims to introduce the recent advances in the purification strategies. Processes like three phase partitioning, ion exchange chromatography, gel filtration, SDS PAGE and denaturation are being discussed. The most powerful of these methods, affinity chromatography also called affinity purification, is dealing with a length. Beneficial role of lectins is envisaged, especially in isolation and characterization of glycoproteins for histochemical analysis.
There is little doubt that proteomics (the study of an organism’s entire complement of proteins) will have a significant influence in all areas of the life sciences in the years to come. The chapter on Phosphoproteomics enlightens the way to distinguish almost all phosphorylated proteins in a cell or tissue at a given time.
We hope that the current volume will be a worthwhile resource for scientists (bio/protein-chemists) with an interest in protein purification or those students who are heading towards implementing purification techniques in their research career. It provides the necessary background material from which to build a strong foundation of knowledge in the field of protein biochemistry.

Rizwan Ahmad, PhD
Department of Biochemistry,
Human Function Group, Oman Medical College, Sohar,
Oman
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CONTENTS :


 1 The Art of Protein Purification 1 William Ward

 2 The Isolation of Invertase from Baker’s Yeast – An Introduction to Protein Purification Strategies 29 Anthony P. Timerman

 3 Protein Purification by Affinity Chromatography 53 Luana C. B. B. Coelho, Andréa F. S. Santos, Thiago H. Napoleão, Maria T. S. Correia and Patrícia M. G. Paiva

 4 Episomal Vectors for Rapid Expression and Purification of Proteins in Mammalian Cells 73
Giovanni Magistrelli, Pauline Malinge, Greg Elson and Nicolas Fischer

 5 Purification Systems Based on Bacterial Surface Proteins 89 Tove Boström, Johan Nilvebrant and Sophia Hober

 6 The Denaturing and Renaturing are Critical Steps in the Purification of Recombinant Protein in Prokaryotic System 137 Di Xiang, Yan Yu and Wei Han

 7 Identification and Characterization of Feruloyl Esterases Produced by Probiotic Bacteria 151 Kin-Kwan Lai, Clara Vu, Ricardo B. Valladares, Anastasia H. Potts and Claudio F. Gonzalez

 8 Lectins: To Combat Infections 167 Barira Islam and Asad U. Khan

 9 Phosphoproteomics 189 Francesco Lonardoni and Alessandra Maria Bossi .




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