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GEL ELECTROPHORESIS – ADVANCED TECHNIQUES

TEXTBOOK : GEL ELECTROPHORESIS – ADVANCED TECHNIQUES

Edited by Sameh Magdeldin .

512 pages .
Open Access .
ISBN 978-953-51-0457-5 .


Since the first report about gel electrophoresis, released in 1930, and despite the alternative fractionation technologies that have recently emerged, gel electrophoresis remains the most popular and most powerful technique that provides a perfect fractionation of macromolecules (DNA, RNA and Proteins). Hundreds of techniques could be set according to experimental atmosphere, including various substrates (agarose or polyacrylamide), gel conditions (native or denaturating), buffers (TAE, TBE, etc) and others. This broad variability enabled further progression and development of gel-based fractionation such as isoelectric focusing, zymograms and 2D-PAGE. With wide application ranging from restriction mapping and genetic fingerprinting, to forensic application and co-application with blotting system, there is no doubt that gel electrophoresis made an overwhelming progress in the “OMICS” era.
It is my pleasure to introduce the book “Gel Electrophoresis - Advanced Techniques”. This book presents a wide research application of this sophisticated technique with wider scope to better cover this popular topic.

Sameh Magdeldin, MVSc, PhD (Physiology), PhD (Proteomics)
Senior post doc researcher and Proteomics team leader
Medical School, Niigata University, Japan
Assistant Professor (Lecturer), Physiology Department
Suez Canal University
Egypt
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CONTENTS : 


Part 1 Electrophoresis Application in Ecological and Biotechnological Aspects .


 1 Application of Gel Electrophoresis Techniques to the Study of Wine Yeast and to Improve Winemaking 3 María Esther Rodríguez, Laureana Rebordinos, Eugenia Muñoz-Bernal, Francisco Javier Fernández-Acero and Jesús Manuel Cantoral

 2 Proteomics in Seaweeds: Ecological Interpretations 21 Loretto Contreras-Porcia and Camilo López-Cristoffanini

 3 Gel Electrophoresis Based Genetic Fingerprinting Techniques on Environmental Ecology 51 Zeynep Cetecioglu, Orhan Ince and Bahar Ince

 4 Gel Electrophoresis of Grapevine (Vitis vinifera L.) Isozymes – A Review 67 Gizella Jahnke, János Májer and János Remete

 5 Molecular Electrophoretic Technique for Authentication of the Fish Genetic Diversity 83 Tsai-Hsin Chiu, Yi-Cheng Su, Hui-Chiu Lin and Chung-Kang Hsu

 6 Gel Electrophoresis for Investigating Enzymes with Biotechnological Application 97 Maria de Lourdes T. M. Polizeli, Simone C. Peixoto-Nogueira, Tony M. da Silva, Alexandre Maller and Hamilton Cabral

Part 2 Electrophoresis Application in Bacteriology, Parasitology, Mycology and Public Health .


 7 Application of Molecular Typing Methods to the Study of Medically Relevant Gram-Positive Cocci 113 Laura Bonofiglio, Noella Gardella and Marta Mollerach

 8 Molecular Microbiology Applied to the Study of Phytopathogenic Fungi 139 Carlos Garrido, Francisco J. Fernández-Acero, María Carbú, Victoria E. González-Rodríguez, Eva Liñeiro and Jesús M. Cantoral

 9 Molecular and Proteolytic Profiles of Trypanosoma cruzi Sylvatic Isolates from Rio de Janeiro-Brazil 157 Suzete A. O. Gomes, Danielle Misael, Cristina S. Silva, Denise Feder, Alice H. Ricardo-Silva, André L. S. Santos, Jacenir R. Santos-Mallet and Teresa Cristina M. Gonçalves

 10 Pulsed Field Gel Electrophoresis in Molecular Typing and Epidemiological Detection of Methicillin Resistant Staphylococcus aureus (MRSA) 179 Velazquez-Meza Maria Elena, Vázquez-Larios Rosario, Hernández Dueñas Ana Maria and Rivera Martínez Eduardo

 11 Usefulness of Pulsed Field Gel Electrophoresis Assay in the Molecular Epidemiological Study of Extended Spectrum Beta Lactamase Producers 193 Patrick Eberechi Akpaka and Padman Jayaratne

Part 3 Electrophoresis Application in the Analysis of Protein-Nucleic Acid Interaction and Chromosomal Replication .


 12 Electrophoretic Mobility Shift Assay: Analyzing Protein – Nucleic Acid Interactions 205 Carolina Alves and Celso Cunha

 13 Analysis of Chromosomal Replication Progression by Gel Electrophoresis 229 Elena C. Guzmán and Enrique Viguera

Part 4 Electrophoresis Application in Enzymology .


 14 Polyacrylamide Gel Electrophoresis an Important Tool for the Detection and Analysis of Enzymatic Activities by Electrophoretic Zymograms 247 Reyna Lucero Camacho Morales, Vanesa Zazueta-Novoa, Carlos A. Leal-Morales, Alberto Flores Martínez, Patricia Ponce Noyola and Roberto Zazueta-Sandoval

 15 Applications of Zymography (Substrate-SDS-PAGE) for Peptidase Screening in a Post-Genomic Era 265 Claudia M. d’Avila-Levy, André L. S. Santos, Patrícia Cuervo, José Batista de Jesus and Marta H. Branquinha

Part 5 Temporal Temperature Gel Electrophoresis .


 16 Temporal Temperature Gel Electrophoresis to Survey Pathogenic Bacterial Communities: The Case of Surgical Site Infections 291 Romano-Bertrand Sara, Parer Sylvie, Lotthé Anne, Colson Pascal, Albat Bernard and Jumas-Bilak Estelle

Part 6 Two-Dimensional Gel Electrophoresis (2-DE) .


 17 Two-Dimensional Gel Electrophoresis Reveals Differential Protein Expression Between Individual Daphnia 315 Darren J. Bauer, Gary B. Smejkal and W. Kelley Thomas

 18 Two-Dimensional Gel Electrophoresis and Mass Spectrometry in Studies of Nanoparticle-Protein Interactions 327 Helen Karlsson, Stefan Ljunggren, Maria Ahrén, Bijar Ghafouri, Kajsa Uvdal, Mats Lindahl and Anders Ljungman

 19 Two Dimensional Gel Electrophoresis in Cancer Proteomics 359 Soundarapandian Kannan, Mohanan V. Sujitha, Shenbagamoorthy Sundarraj and Ramasamy Thirumurugan

Part 7 Other Applications of Gel Electrophoresis Technique .


 20 Enzymatic Staining for Detection of Phenol-Oxidizing Isozymes Involved in Lignin- Degradation by Lentinula edodes on Native-PAGE 393 Eiji Tanesaka, Naomi Saeki, Akinori Kochi and Motonobu Yoshida

 21 Protection Studies by Antioxidants Using Single Cell Gel Electrophoresis (Comet Assay) 413 Pınar Erkekoglu

 22 Gel Electrophoresis as Quality Control Method of the Radiolabeled Monoclonal Antibodies 447 Veronika Kocurová

 23 Gel Electrophoresis as a Tool to Study Polymorphism and Nutritive Value of the Seed Storage Proteins in the Grain Sorghum 463 Lev Elkonin, Julia Italianskaya and Irina Fadeeva

 24 Extraction and Electrophoresis of DNA from the Remains of Mexican Ancient Populations 479 Maria de Lourdes Muñoz, Mauro Lopez-Armenta, Miguel Moreno-Galeana, Alvaro Díaz-Badillo,
Gerardo Pérez-Ramirez, Alma Herrera-Salazar, Elizabeth Mejia-Pérez-Campos, Sergio Gómez-Chávez and Adrián Martínez-Meza .




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